The fatal crash occurred around 1:45pm near the Go Wholesale Rawai branch. Chalong Police Station received the emergency report from its radio centre and dispatched officers to the scene.
Officers from the Chalong Police and rescue workers arrived to find the woman, Nantanapat Phengchit, 31, from Songkhla, lying unconscious in the middle of the road with serious injuries.
A black Honda PCX motorcycle registered in Surat Thani lay damaged beside her.
Despite the efforts of medical staff from Chalong Hospital, who performed CPR upon arrival, Ms Nantanapat was pronounced dead at the scene. Her body was later transported to Vachira Phuket Hospital.
The motorbike was being driven by Rattanaporn Chaisongkhram, from Phang Nga, who suffered minor injuries. She was treated at the scene before being taken to Chalong Hospital for further medical examination.
The truck involved in the incident was a white six-wheel Hino, registered in Phuket, driven by Aree Hansuek, a resident of Thalang. He remained at the scene and cooperated with authorities.
Initial CCTV footage reviewed by investigators showed the motorcycle travelling in the left lane along Wiset Rd toward the Chalong Circle.
The footage showed the truck following and then attempting to overtake the motorcycle, resulting in a collision that caused both riders to fall, said police.
Police are continuing their investigation to determine the exact cause of the crash and will proceed with legal action as appropriate.